Staff - Non Union

Job Category

Non Union Technicians and Research Assistants

Job Profile

Non Union Salaried - Research Assistant /Technician 3

Job Title

Cell Culture Technician (Vallance Lab)

Department

Vallance Laboratory | Department of Paediatrics | Faculty of Medicine

Compensation Range

$3,982.67 - $4,703.83 CAD Monthly

Posting End Date

January 16, 2024

Note: Applications will be accepted until 11:59 PM on the day prior to the Posting End Date above.

Job End Date

Jan 31, 2024

This position is located within a health-care facility, therefore, the successful candidate will be required to provide verification of full vaccination against Covid-19 provided prior to the start date, as required by a provincial health mandate.

This offer is conditional upon successful completion of a Criminal Record Check.

Job Summary

The Vallance lab is a world leading research group that studies inflammatory bowel disease as well as infectious causes of intestinal inflammation. In particular, the Vallance lab focuses on bacteria - host interactions at the intestinal mucosal interface. We are looking for a motivated and talented individual to join our team as a cell culture technician. The successful candidate will work on cutting-edge projects using cell culture models to develop novel therapies for Inflammatory Bowel Disease. Specifically, the successful candidate should possess significant cell culture expertise as well as techniques useful in assessing mammalian cell function.

Among their roles, the candidate will be trained to generate, culture and maintain patient-derived intestinal organoids, as well as assess their functions using routine standardized assays. The successful candidate will assist senior staff and trainees in growing and analyzing organoids, using modern techniques, performed under minimal supervision. The cell culture technician role requires extensive laboratory and cell culture experience. It is expected that the successful candidate will master specific technical skills underlying primary cell culture technique, and be sufficiently skilled to independently perform such work and instruct others as well. The role will also require the successful candidate to work closely with an interdisciplinary team to tackle cutting-edge challenges in organoid research.

ORGANIZATIONAL STATUS

The cell culture technician will report directly to Dr. Bruce Vallance and will liaise with other members of Dr. Vallance's research group, including co-investigators, researchers, research assistants, post-doctoral fellows, students and staff.

WORK PERFORMED
• Culture epithelial cell lines, as well as process intestinal biopsies for organoid isolation derived from patient samples and maintain collection records
• Oversee the daily operation of the lab’s cell culture work, as well as maintain organoid cultures including culturing and passaging of intestinal organoid cultures. Also prepare organoid media and perform routine testing to identify possible contamination.
• Perform routine standardized assays to evaluate organoid quality and function
• Assist in the development of protocols for organoid culture and experiment
• Maintain inventory of frozen organoid stocks
• Plan and perform experiments; troubleshoot problems; collect, record, analyze and interpret experimental results
• Maintain a detailed lab notebook
• Provide training to undergraduate students, as well as new and lower level technicians in the use of equipment, techniques and procedures.
• Set up, test, operate and maintain common laboratory equipment and laboratory reagents.